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close orthologs  (ATCC)


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    Structured Review

    ATCC close orthologs
    Relationship of RbCas12a to other Cas12a <t>orthologs.</t> ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved <t>(HkCas12a,</t> Helcococcus kunzii ATCC 51366; <t>CeCas12a,</t> Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; <t>LpCas12a,</t> Lachnospira pectinoschiza strain <t>2789STDY5834886;</t> EeCas12a, Lachnospira eligens ATCC 27750; <t>AsCas12a,</t> Acidaminococcus sp. <t>BV36L;</t> FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.
    Close Orthologs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii"

    Article Title: Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms23169289

    Relationship of RbCas12a to other Cas12a orthologs. ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved (HkCas12a, Helcococcus kunzii ATCC 51366; CeCas12a, Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; LpCas12a, Lachnospira pectinoschiza strain 2789STDY5834886; EeCas12a, Lachnospira eligens ATCC 27750; AsCas12a, Acidaminococcus sp. BV36L; FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.
    Figure Legend Snippet: Relationship of RbCas12a to other Cas12a orthologs. ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved (HkCas12a, Helcococcus kunzii ATCC 51366; CeCas12a, Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; LpCas12a, Lachnospira pectinoschiza strain 2789STDY5834886; EeCas12a, Lachnospira eligens ATCC 27750; AsCas12a, Acidaminococcus sp. BV36L; FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.

    Techniques Used: Sequencing



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    Relationship of RbCas12a to other Cas12a <t>orthologs.</t> ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved <t>(HkCas12a,</t> Helcococcus kunzii ATCC 51366; <t>CeCas12a,</t> Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; <t>LpCas12a,</t> Lachnospira pectinoschiza strain <t>2789STDY5834886;</t> EeCas12a, Lachnospira eligens ATCC 27750; <t>AsCas12a,</t> Acidaminococcus sp. <t>BV36L;</t> FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.
    Close Orthologs, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Relationship of RbCas12a to other Cas12a <t>orthologs.</t> ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved <t>(HkCas12a,</t> Helcococcus kunzii ATCC 51366; <t>CeCas12a,</t> Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; <t>LpCas12a,</t> Lachnospira pectinoschiza strain <t>2789STDY5834886;</t> EeCas12a, Lachnospira eligens ATCC 27750; <t>AsCas12a,</t> Acidaminococcus sp. <t>BV36L;</t> FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.
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    Image Search Results


    Relationship of RbCas12a to other Cas12a orthologs. ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved (HkCas12a, Helcococcus kunzii ATCC 51366; CeCas12a, Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; LpCas12a, Lachnospira pectinoschiza strain 2789STDY5834886; EeCas12a, Lachnospira eligens ATCC 27750; AsCas12a, Acidaminococcus sp. BV36L; FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.

    Journal: International Journal of Molecular Sciences

    Article Title: Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii

    doi: 10.3390/ijms23169289

    Figure Lengend Snippet: Relationship of RbCas12a to other Cas12a orthologs. ( A ) Multiple sequence alignments of RbCpf1 with common orthologs of 18 Cas12a proteins that function in human cells. Catalytic Asp1194 and Glu1290 residues (numeration shown here for total alignment) are conserved (HkCas12a, Helcococcus kunzii ATCC 51366; CeCas12a, Coprococcus eutactus sp.; ErCas12a, Eubacterium rectale sp.; ArCas12a, Agathobacter rectalis strain 2789STDY5834884; LpCas12a, Lachnospira pectinoschiza strain 2789STDY5834886; EeCas12a, Lachnospira eligens ATCC 27750; AsCas12a, Acidaminococcus sp. BV36L; FnCas12a, Francisella novicida U112; TsCas12a, Thiomicrospira sp. XS5; Mb3Cas12a, Moraxella bovoculi sp.; Mb2Cas12a, Moraxella bovoculi AAX08_00205; MbCas12a, Moraxella bovoculi 237; RbCas12a, Ruminococcus bromii sp.; LbCas12a, Lachnospiraceae bacterium ND2006; PxCas12a, Pseudobutyrivibrio xylanivorans strain DSM 10317; PrCas12a, Pseudobutyrivibrio ruminis CF1b; BfCas12a, Butyrivibrio fibrisolvens MD2001; Lb2Cas12a, Lachnospiraceae bacterium MA2020; BsCas12a, Butyrivibrio sp. NC3005). ( B ) Neighbor-joining tree without distance corrections.

    Article Snippet: In total, 18 Cas12a proteins functioning in mammalian cells and their close orthologs (HkCas12a, Helcococcus kunzii ATCC 51366; AsCas12a, Acidaminococcus sp. BV36L; LpCas12a, Lachnospira pectinoschiza strain 2789STDY5834886; CeCas12a, Coprococcus eutactus sp .

    Techniques: Sequencing